菲胁迫下蒿柳抗氧化系统的响应
Response of antioxidant system of Salix viminalis under PAHs phenanthrene stress
投稿时间:2019-07-29  修订日期:2019-12-23
DOI:
中文关键词:  蒿柳    活性氧  抗氧化物质
英文关键词:Salix viminalis  Phenanthrene  Reactive oxygen  Antioxidants
基金项目:中央级公益性科研院所基本科研业务费专项基金(CAFYBB2018ZB002);湖南省林业科技计划项目“基于重金属污染植物修复的灌木能源柳引种与评价(XLK201834)。
作者单位E-mail
李霞 中国林业科学研究院林业研究所 393362284@qq.com 
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中文摘要:
      [目的] 研究菲胁迫下活性氧和抗氧化物质的变化规律,探究蒿柳抗氧化系统的防御机制,为提高其对多环芳烃(PAHs)的抗性及加强植物修复的研究提供理论依据。[方法] 以蒿柳扦插苗为试验材料,采用水培的方式,研究其在0和1.0mg·L-1菲处理下活性氧、抗氧化酶、抗氧化剂以及丙二醛(MDA)的动态变化,处理时间为16d。[结果] 研究表明:(1)菲处理后H2O2含量和氧自由基(O2·-)生成速率迅速增加,MDA含量升高。过氧化氢酶(CAT)活性在第4d即显著上升,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性在第8d开始显著升高。第16d时H2O2含量下降到与对照无显著差异,O2·-和MDA的增加量下降。(2)还原型谷胱甘肽(GSH)和谷胱甘肽还原酶(GR)在处理后第4d即迅速上升,谷胱甘肽-s-转移酶(GST)呈缓慢上升趋势。(3)还原型抗坏血酸(AsA)含量一开始低于对照,但随着处理时间的延长呈上升趋势,在第16d时高于对照。[结论] 菲胁迫下,O2·-是造成细胞膜脂过氧化的主要活性氧。SOD活性一直高于对照,但不足以清除增加的O2·-,CAT和POD的升高可以清除过量的H2O2。GSH通过GST的催化参与菲的解毒,是抵御PAHs胁迫的有效抗氧化剂。
英文摘要:
      [objective] The dynamic changes of reactive oxygen and antioxidant substances under phenanthrene stress were studied to explore the defense mechanism of the antioxidant system of S. viminalis, and this provided theoretical basis for improving resistance of plants to PAHs stress and enhancing phytoremediation potential. [method] In this study, cutting seedlings of S. viminalis was selected as experimental materials, and a 16-day hydroponic experiment with 0 and 1.0mg·L-1 phenanthrene concentrations were conducted to study the dynamic changes of reactive oxygen, antioxidant enzymes, antioxidants and malondialdehyde (MDA) contents. [results] Results showed that: (1) Under phenanthrene treatment, superoxide anion radical(O2·-) production and H2O2 content increased rapidly, then MDA content rose. Catalase(CAT) activity significantly increased at 4d, and superoxide dismutase (SOD) and peroxidase(POD) activity increased at 8d. At 16d, there was no significant difference between the H2O2 content with phenanthrene treatment and that of the control. Meanwhile, the increment of O2·- and MDA also decreased. (2) Reduced glutathione(GSH) and glutathione reductase(GR) contents rose rapidly at 4d under phenanthrene treatment, and the activity of glutathione-s-transferase(GST) increased slowly. (3) Under phenanthrene treatment, the content of ascorbic acid (AsA) was initially lower than the control group, but was higher than it at 16d as the increasing. [conclusion] Under phenanthrene stress, O2·- was the main reactive oxygen that caused cell membrane lipid peroxidation. SOD activity was always higher than that of the control group, but not enough to eliminate the increased O2·-. The increase of CAT and POD could eliminate the excessive H2O2. GSH participated in the detoxification of phenanthrene through the catalysis of GST. Hence, it was an effective antioxidant to resist the stress of PAHs.
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