吕品,亓倩,杨璞,陈航,陈晓鸣.基于16S测序分析白蜡虫二龄雌雄若虫共生菌的多样性分析[J].林业科学研究,2019,32(5):34-41
基于16S测序分析白蜡虫二龄雌雄若虫共生菌的多样性分析
Analysis on Structure and Diversity of Bacterial Community of the Second-instar Ericerus pela Nymph Based on 16S rRNA High-throughput Sequencing
投稿时间:2018-10-11  修订日期:2019-06-25
DOI:10.13275/j.cnki.lykxyj.2019.05.005
中文关键词:  白蜡虫  二龄若虫  16S rDNA  昆虫共生细菌  立克次氏体
英文关键词:Ericerus pela  second-instar nymph  16S rRNA  symbiotic bacteria  Rickettsia
基金项目:中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金重点项目(CAFYBB2017ZB005);国家林业局948项目(2014-4-66)
作者单位E-mail
吕品 1 中国林业科学研究院资源昆虫研究所, 国家林业和草原局资源昆虫培育与利用重点实验室, 云南 昆明 650224  
亓倩 1 中国林业科学研究院资源昆虫研究所, 国家林业和草原局资源昆虫培育与利用重点实验室, 云南 昆明 650224  
杨璞 1 中国林业科学研究院资源昆虫研究所, 国家林业和草原局资源昆虫培育与利用重点实验室, 云南 昆明 650224  
陈航 1 中国林业科学研究院资源昆虫研究所, 国家林业和草原局资源昆虫培育与利用重点实验室, 云南 昆明 650224  
陈晓鸣 1 中国林业科学研究院资源昆虫研究所, 国家林业和草原局资源昆虫培育与利用重点实验室, 云南 昆明 650224 cafcxm@139.com 
摘要点击次数: 90
全文下载次数: 174
中文摘要:
      [目的]探明白蜡虫二龄雌雄若虫共生菌的种类和结构。[方法]利用高通量测序技术,对白蜡虫二龄雌雄虫16S rRNA的V3-V4区进行高通量测序,使用Uparse软件对有效标签进行聚类,使用Mothur方法与SILVA的SSUrRNA数据库进行物种注释,分析雌雄间共生菌物种丰度及多样性的差异。[结果]共得到了1 798 646条有效标签,以97%相似度为标准进行聚类分析,得到1 334个OTUs,其中,有14个门,29个纲,60个目,109个科,165个属,55个种得到注释。与其他昆虫明显不同,白蜡虫二龄若虫中立克次氏体属为优势菌(雌85.740%,雄95.462%),缺乏布赫纳氏菌和沃尔巴克氏体。[结论]白蜡虫与其它昆虫的共生菌有很大差异,立克次氏体在白蜡虫二龄若虫中占绝对优势,具有固氮作用的根瘤菌目和芽孢杆菌目、能合成类胡萝卜素的鞘脂单胞菌目为次优势菌,在共生菌中占有一定比例。白蜡虫若虫共生菌独特的现象可能与白蜡虫独特的生物习性、生态学特征和营养相关。
英文摘要:
      [Objective] To investigate the variation and diversity of symbiotic bacteria both in the second-instar male and female of Ericerus pela.[Method] The V3-V4 region of the 16S rRNA was performed using high-throughput sequencing technology. Uparse software was used to cluster the qualified labels. Species annotation was taken by using the Mothur and SILVA's SSUrRNA database to analysis the differences in species abundance and diversity between male and female symbiotic bacteria.[Result] There were 1798646 qualified labels identified in this experiment. Cluster analysis was performed with 97% similarity as the standard, and 1 334 OTUs (operational taxonomic unit) were obtained. After the annotation, 14 phylums, 29 classes, 60 orders, 109 families, 165 genera, and 55 species were obtained. E. pela is significantly different from the other insects. For the second-instar nymph, Rickettsia (85.740% in female, 95.462% in male) was the dominant bacteria while Buchnera and Wolbachia were absent. A total of 386 symbiotic bacteria were identified in the second-instar nymphs.[Conclusion] The endosymbiotic bacteria of E. pela are quite different from that of other insects. Rickettsia has an absolute advantage in the second-instar nymph of E. pela. Rhizobiales and Bacillales with nitrogen fixation, Sphingomonadales which can synthesize carotenoids, are the subdominant bacteria and occupy a certain proportion in symbiotic bacteria. The unique phenomenon of the E. pela symbiotic bacteria may be related to its unique biological habits, ecological characteristics and nutrition.
查看全文  查看/发表评论  下载PDF阅读器
关闭
京ICP备09064894号-3
版权所有:《林业科学研究》编辑部
地址:北京颐和园后中国林科院林业所 电话:010-62889680 E-mail:lykxyj@vip.163.com
技术支持:北京勤云科技发展有限公司